Tissue-specific expression conferred by the S-adenosyl-L-methionine synthetase promoter of Arabidopsis thaliana in transgenic poplar.
Identifieur interne : 004A26 ( Main/Exploration ); précédent : 004A25; suivant : 004A27Tissue-specific expression conferred by the S-adenosyl-L-methionine synthetase promoter of Arabidopsis thaliana in transgenic poplar.
Auteurs : K. Vander Mijnsbrugge [Belgique] ; M. Van Montagu ; D. Inzé ; W. BoerjanSource :
- Plant & cell physiology [ 0032-0781 ] ; 1996.
Descripteurs français
- KwdFr :
- Adémétionine (métabolisme), Arabidopsis (enzymologie), Arabidopsis (génétique), Arbres (MeSH), Biosynthèse des protéines (MeSH), Expression des gènes (MeSH), Facteurs temps (MeSH), Glucuronidase (métabolisme), Maturation post-transcriptionnelle des ARN (MeSH), Methionine adenosyltransferase (génétique), Methionine adenosyltransferase (métabolisme), Protéines de fusion recombinantes (génétique), Protéines végétales (génétique), Régions promotrices (génétique) (MeSH), Transformation génétique (MeSH), Végétaux génétiquement modifiés (MeSH).
- MESH :
- enzymologie : Arabidopsis.
- génétique : Arabidopsis, Methionine adenosyltransferase, Protéines de fusion recombinantes, Protéines végétales.
- métabolisme : Adémétionine, Glucuronidase, Methionine adenosyltransferase.
- Arbres, Biosynthèse des protéines, Expression des gènes, Facteurs temps, Maturation post-transcriptionnelle des ARN, Régions promotrices (génétique), Transformation génétique, Végétaux génétiquement modifiés.
English descriptors
- KwdEn :
- Arabidopsis (enzymology), Arabidopsis (genetics), Gene Expression (MeSH), Glucuronidase (metabolism), Methionine Adenosyltransferase (genetics), Methionine Adenosyltransferase (metabolism), Plant Proteins (genetics), Plants, Genetically Modified (MeSH), Promoter Regions, Genetic (MeSH), Protein Biosynthesis (MeSH), RNA Processing, Post-Transcriptional (MeSH), Recombinant Fusion Proteins (genetics), S-Adenosylmethionine (metabolism), Time Factors (MeSH), Transformation, Genetic (MeSH), Trees (MeSH).
- MESH :
- chemical , genetics : Methionine Adenosyltransferase, Plant Proteins, Recombinant Fusion Proteins.
- chemical , metabolism : Glucuronidase, Methionine Adenosyltransferase, S-Adenosylmethionine.
- enzymology : Arabidopsis.
- genetics : Arabidopsis.
- Gene Expression, Plants, Genetically Modified, Promoter Regions, Genetic, Protein Biosynthesis, RNA Processing, Post-Transcriptional, Time Factors, Transformation, Genetic, Trees.
Abstract
In Arabidopsis the promoter of the gene encoding S-adenosyl-L-methionine synthetase (SAM-S) Psam-1 confers expression preferentially in the vascular tissue. In search for promoters that drive expression in particular cells of the lignifying tissues in trees, we have analyzed the expression pattern conferred by the Psam-1 promoter in transgenic poplar. Histochemical analyses demonstrated beta-glucuronidase (GUS) activity mainly in phloem and cortex tissue throughout the plant, and in root tips. Fluorimetric assays showed high GUS activity in the tissues outside (phloem, cortex and cork) compared to those inside (xylem and pith) of the cambial layer. In contrast, the endogenous SAM-S activity was high in tissues inside and low in tissues outside of the cambial layer. RNA gel blot analysis demonstrated a high transcript level of the endogenous sam-s gene(s) in tissues both outside and inside the cambial layer. This indicates that the low SAM-S activity in the bark was at least partially due to translational and/or post-translational regulation of the endogenous sam-s gene(s). In dormant transgenics, the tissue specificity was conserved, but the activity levels were up to 10-fold reduced.
DOI: 10.1093/oxfordjournals.pcp.a029061
PubMed: 9032966
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Tissue-specific expression conferred by the S-adenosyl-L-methionine synthetase promoter of Arabidopsis thaliana in transgenic poplar.</title><author><name sortKey="Vander Mijnsbrugge, K" sort="Vander Mijnsbrugge, K" uniqKey="Vander Mijnsbrugge K" first="K" last="Vander Mijnsbrugge">K. Vander Mijnsbrugge</name><affiliation wicri:level="4"><nlm:affiliation>Department of Genetics, Flanders Interuniversity Institute for Biotechnology, Universiteit Gent, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>Department of Genetics, Flanders Interuniversity Institute for Biotechnology, Universiteit Gent</wicri:regionArea><placeName><settlement type="city">Gand</settlement><region>Région flamande</region><region type="district" nuts="2">Province de Flandre-Orientale</region></placeName><orgName type="university">Université de Gand</orgName></affiliation></author><author><name sortKey="Van Montagu, M" sort="Van Montagu, M" uniqKey="Van Montagu M" first="M" last="Van Montagu">M. Van Montagu</name></author><author><name sortKey="Inze, D" sort="Inze, D" uniqKey="Inze D" first="D" last="Inzé">D. Inzé</name></author><author><name sortKey="Boerjan, W" sort="Boerjan, W" uniqKey="Boerjan W" first="W" last="Boerjan">W. Boerjan</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1996">1996</date><idno type="RBID">pubmed:9032966</idno><idno type="pmid">9032966</idno><idno type="doi">10.1093/oxfordjournals.pcp.a029061</idno><idno type="wicri:Area/Main/Corpus">004A17</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">004A17</idno><idno type="wicri:Area/Main/Curation">004A17</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">004A17</idno><idno type="wicri:Area/Main/Exploration">004A17</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Tissue-specific expression conferred by the S-adenosyl-L-methionine synthetase promoter of Arabidopsis thaliana in transgenic poplar.</title><author><name sortKey="Vander Mijnsbrugge, K" sort="Vander Mijnsbrugge, K" uniqKey="Vander Mijnsbrugge K" first="K" last="Vander Mijnsbrugge">K. Vander Mijnsbrugge</name><affiliation wicri:level="4"><nlm:affiliation>Department of Genetics, Flanders Interuniversity Institute for Biotechnology, Universiteit Gent, Belgium.</nlm:affiliation><country xml:lang="fr">Belgique</country><wicri:regionArea>Department of Genetics, Flanders Interuniversity Institute for Biotechnology, Universiteit Gent</wicri:regionArea><placeName><settlement type="city">Gand</settlement><region>Région flamande</region><region type="district" nuts="2">Province de Flandre-Orientale</region></placeName><orgName type="university">Université de Gand</orgName></affiliation></author><author><name sortKey="Van Montagu, M" sort="Van Montagu, M" uniqKey="Van Montagu M" first="M" last="Van Montagu">M. Van Montagu</name></author><author><name sortKey="Inze, D" sort="Inze, D" uniqKey="Inze D" first="D" last="Inzé">D. Inzé</name></author><author><name sortKey="Boerjan, W" sort="Boerjan, W" uniqKey="Boerjan W" first="W" last="Boerjan">W. Boerjan</name></author></analytic><series><title level="j">Plant & cell physiology</title><idno type="ISSN">0032-0781</idno><imprint><date when="1996" type="published">1996</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Arabidopsis (enzymology)</term><term>Arabidopsis (genetics)</term><term>Gene Expression (MeSH)</term><term>Glucuronidase (metabolism)</term><term>Methionine Adenosyltransferase (genetics)</term><term>Methionine Adenosyltransferase (metabolism)</term><term>Plant Proteins (genetics)</term><term>Plants, Genetically Modified (MeSH)</term><term>Promoter Regions, Genetic (MeSH)</term><term>Protein Biosynthesis (MeSH)</term><term>RNA Processing, Post-Transcriptional (MeSH)</term><term>Recombinant Fusion Proteins (genetics)</term><term>S-Adenosylmethionine (metabolism)</term><term>Time Factors (MeSH)</term><term>Transformation, Genetic (MeSH)</term><term>Trees (MeSH)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adémétionine (métabolisme)</term><term>Arabidopsis (enzymologie)</term><term>Arabidopsis (génétique)</term><term>Arbres (MeSH)</term><term>Biosynthèse des protéines (MeSH)</term><term>Expression des gènes (MeSH)</term><term>Facteurs temps (MeSH)</term><term>Glucuronidase (métabolisme)</term><term>Maturation post-transcriptionnelle des ARN (MeSH)</term><term>Methionine adenosyltransferase (génétique)</term><term>Methionine adenosyltransferase (métabolisme)</term><term>Protéines de fusion recombinantes (génétique)</term><term>Protéines végétales (génétique)</term><term>Régions promotrices (génétique) (MeSH)</term><term>Transformation génétique (MeSH)</term><term>Végétaux génétiquement modifiés (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Methionine Adenosyltransferase</term><term>Plant Proteins</term><term>Recombinant Fusion Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Glucuronidase</term><term>Methionine Adenosyltransferase</term><term>S-Adenosylmethionine</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Arabidopsis</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>Arabidopsis</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Arabidopsis</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Arabidopsis</term><term>Methionine adenosyltransferase</term><term>Protéines de fusion recombinantes</term><term>Protéines végétales</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Adémétionine</term><term>Glucuronidase</term><term>Methionine adenosyltransferase</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Gene Expression</term><term>Plants, Genetically Modified</term><term>Promoter Regions, Genetic</term><term>Protein Biosynthesis</term><term>RNA Processing, Post-Transcriptional</term><term>Time Factors</term><term>Transformation, Genetic</term><term>Trees</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Arbres</term><term>Biosynthèse des protéines</term><term>Expression des gènes</term><term>Facteurs temps</term><term>Maturation post-transcriptionnelle des ARN</term><term>Régions promotrices (génétique)</term><term>Transformation génétique</term><term>Végétaux génétiquement modifiés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">In Arabidopsis the promoter of the gene encoding S-adenosyl-L-methionine synthetase (SAM-S) Psam-1 confers expression preferentially in the vascular tissue. In search for promoters that drive expression in particular cells of the lignifying tissues in trees, we have analyzed the expression pattern conferred by the Psam-1 promoter in transgenic poplar. Histochemical analyses demonstrated beta-glucuronidase (GUS) activity mainly in phloem and cortex tissue throughout the plant, and in root tips. Fluorimetric assays showed high GUS activity in the tissues outside (phloem, cortex and cork) compared to those inside (xylem and pith) of the cambial layer. In contrast, the endogenous SAM-S activity was high in tissues inside and low in tissues outside of the cambial layer. RNA gel blot analysis demonstrated a high transcript level of the endogenous sam-s gene(s) in tissues both outside and inside the cambial layer. This indicates that the low SAM-S activity in the bark was at least partially due to translational and/or post-translational regulation of the endogenous sam-s gene(s). In dormant transgenics, the tissue specificity was conserved, but the activity levels were up to 10-fold reduced.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">9032966</PMID><DateCompleted><Year>1997</Year><Month>03</Month><Day>25</Day></DateCompleted><DateRevised><Year>2019</Year><Month>05</Month><Day>12</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0032-0781</ISSN><JournalIssue CitedMedium="Print"><Volume>37</Volume><Issue>8</Issue><PubDate><Year>1996</Year><Month>Dec</Month></PubDate></JournalIssue><Title>Plant & cell physiology</Title><ISOAbbreviation>Plant Cell Physiol</ISOAbbreviation></Journal><ArticleTitle>Tissue-specific expression conferred by the S-adenosyl-L-methionine synthetase promoter of Arabidopsis thaliana in transgenic poplar.</ArticleTitle><Pagination><MedlinePgn>1108-15</MedlinePgn></Pagination><Abstract><AbstractText>In Arabidopsis the promoter of the gene encoding S-adenosyl-L-methionine synthetase (SAM-S) Psam-1 confers expression preferentially in the vascular tissue. In search for promoters that drive expression in particular cells of the lignifying tissues in trees, we have analyzed the expression pattern conferred by the Psam-1 promoter in transgenic poplar. Histochemical analyses demonstrated beta-glucuronidase (GUS) activity mainly in phloem and cortex tissue throughout the plant, and in root tips. Fluorimetric assays showed high GUS activity in the tissues outside (phloem, cortex and cork) compared to those inside (xylem and pith) of the cambial layer. In contrast, the endogenous SAM-S activity was high in tissues inside and low in tissues outside of the cambial layer. RNA gel blot analysis demonstrated a high transcript level of the endogenous sam-s gene(s) in tissues both outside and inside the cambial layer. This indicates that the low SAM-S activity in the bark was at least partially due to translational and/or post-translational regulation of the endogenous sam-s gene(s). In dormant transgenics, the tissue specificity was conserved, but the activity levels were up to 10-fold reduced.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Vander Mijnsbrugge</LastName><ForeName>K</ForeName><Initials>K</Initials><AffiliationInfo><Affiliation>Department of Genetics, Flanders Interuniversity Institute for Biotechnology, Universiteit Gent, Belgium.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Van Montagu</LastName><ForeName>M</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Inzé</LastName><ForeName>D</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Boerjan</LastName><ForeName>W</ForeName><Initials>W</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Japan</Country><MedlineTA>Plant Cell Physiol</MedlineTA><NlmUniqueID>9430925</NlmUniqueID><ISSNLinking>0032-0781</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011993">Recombinant Fusion Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>7LP2MPO46S</RegistryNumber><NameOfSubstance UI="D012436">S-Adenosylmethionine</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 2.5.1.6</RegistryNumber><NameOfSubstance UI="D008716">Methionine Adenosyltransferase</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.2.1.31</RegistryNumber><NameOfSubstance UI="D005966">Glucuronidase</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D017360" MajorTopicYN="N">Arabidopsis</DescriptorName><QualifierName UI="Q000201" MajorTopicYN="N">enzymology</QualifierName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015870" MajorTopicYN="Y">Gene Expression</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005966" MajorTopicYN="N">Glucuronidase</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008716" MajorTopicYN="N">Methionine Adenosyltransferase</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D030821" MajorTopicYN="N">Plants, Genetically Modified</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011401" MajorTopicYN="Y">Promoter Regions, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014176" MajorTopicYN="N">Protein Biosynthesis</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012323" MajorTopicYN="N">RNA Processing, Post-Transcriptional</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011993" MajorTopicYN="N">Recombinant Fusion Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012436" MajorTopicYN="N">S-Adenosylmethionine</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013997" MajorTopicYN="N">Time Factors</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014170" MajorTopicYN="N">Transformation, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014197" MajorTopicYN="N">Trees</DescriptorName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>1996</Year><Month>12</Month><Day>1</Day></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>1996</Year><Month>12</Month><Day>1</Day><Hour>0</Hour><Minute>1</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>1996</Year><Month>12</Month><Day>1</Day><Hour>0</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">9032966</ArticleId><ArticleId IdType="doi">10.1093/oxfordjournals.pcp.a029061</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Belgique</li></country><region><li>Province de Flandre-Orientale</li><li>Région flamande</li></region><settlement><li>Gand</li></settlement><orgName><li>Université de Gand</li></orgName></list><tree><noCountry><name sortKey="Boerjan, W" sort="Boerjan, W" uniqKey="Boerjan W" first="W" last="Boerjan">W. Boerjan</name><name sortKey="Inze, D" sort="Inze, D" uniqKey="Inze D" first="D" last="Inzé">D. Inzé</name><name sortKey="Van Montagu, M" sort="Van Montagu, M" uniqKey="Van Montagu M" first="M" last="Van Montagu">M. Van Montagu</name></noCountry><country name="Belgique"><region name="Région flamande"><name sortKey="Vander Mijnsbrugge, K" sort="Vander Mijnsbrugge, K" uniqKey="Vander Mijnsbrugge K" first="K" last="Vander Mijnsbrugge">K. Vander Mijnsbrugge</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/PoplarV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 004A26 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 004A26 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= PoplarV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:9032966
|texte= Tissue-specific expression conferred by the S-adenosyl-L-methionine synthetase promoter of Arabidopsis thaliana in transgenic poplar.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:9032966" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a PoplarV1
| This area was generated with Dilib version V0.6.37. |  |